isotype control antibody, mouse igg1 (Miltenyi Biotec)
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isotype control antibody, mouse igg1
Isotype Control Antibody, Mouse Igg1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+controls/Isotype+Control+Antibody%2C+mouse+IgG1/custom%40130-113-761%4042727576
Average 96 stars, based on 206 article reviews
Isotype Control Antibody, Mouse Igg1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+controls/Isotype+Control+Antibody%2C+mouse+IgG1/custom%40130-113-761%4042727576
Average 96 stars, based on 206 article reviews
isotype control antibody, mouse igg1 - by Bioz Stars,
2026-09
96/100 stars
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Staining:Article Title: Immortalized human hair follicle-derived mesenchymal-like stromal cells for the long-term production of scalable Immunomodulatory and regenerative secretome Article Snippet: .. Cells were stained with anti-MHC-I-PeVio770 (Miltenyi Biotec, 130-120-573), anti-MHC-II-VioBlue (Miltenyi Biotec, 130-111-947) or the corresponding Article Title: Expression of Human CEACAM Receptors Promotes Inflammation and Organ Damage During Systemic Candida albicans Infection in Mice Article Snippet: .. Cells were stained and analyzed on an Attune Acoustic Focusing Cytometer (Life Technologies, Thermo Fisher Scientific, Darmstadt, Germany) using the Attune software v2.1, as described in the respective figure legends with the following antibodies or the corresponding Article Title: Single-cell RNA sequencing reveals disease associated changes in brain endothelial cells in the 5XFAD mouse. Article Snippet: After tissue homogenization, dissociation, myelin/debris removal, and red blood cell lysis, cells were incubated with fluorescently conjugated antibodies against CD31 (0.1 μg/mL, Invitrogen 170311-82) and CD45 (0.1 μg/mL, BD Pharmingen 553081) diluted in 0.5% BSA (w/v) (Research Products International A30075100.0) in Dulbecco’s Phosphate-Buffered Saline (DPBS, Gibco 141190-144) for 30 minutes at 4°C. .. After immunolabeling, cells were rinsed with DPBS containing 0.5% BSA and resuspended in DPBS containing 0.5% BSA with 4’6-Diamidino-2-phenylindole (DAPI, 0.2μg/mL, Sigma Aldrich D9542) or without DAPI for single stained compensation controls and Article Title: Single-cell RNA sequencing reveals disease associated changes in brain endothelial cells in the 5XFAD mouse Article Snippet: After tissue homogenization, dissociation, myelin/debris removal, and red blood cell lysis, cells were incubated with fluorescently conjugated antibodies against CD31 (0.1 μg/mL, Invitrogen 170311-82) and CD45 (0.1 μg/mL, BD Pharmingen 553081) diluted in 0.5% BSA (w/v) (Research Products International A30075-100.0) in Dulbecco’s Phosphate-Buffered Saline (DPBS, Gibco 141190-144) for 30 min at 4 °C. .. After immunolabeling, cells were rinsed with DPBS containing 0.5% BSA and resuspended in DPBS containing 0.5% BSA with 4’6-Diamidino-2-phenylindole (DAPI, 0.2 μg/mL, Sigma Aldrich D9542) or without DAPI for single stained compensation controls and Article Title: Immunomolecular and reactivity landscapes of gut IgA subclasses in homeostasis and inflammatory bowel disease. Article Snippet: Sonia Tejedor Vaquero1* , Hadas Neuman2* , Laura Comerma1,5 , Xavi Marcos-Fa1 , Celia Corral-Vazquez1 , Mathieu Uzzan3 , Marc Pybus1 , Daniel Segura-Garzón1 , Joana Guerra1 , Lisa Perruzza4 , Roser Tachó-Piñot1 , Jordi Sintes1 , Adam Rosenstein3 , Emilie K. Grasset3 , Mar Iglesias5 , Monica Gonzalez Farré5 , Joan Lop5 , Maria Evangelina Patriaca-Amiano5 , Monica Larrubia-Loring5 , Pablo Santiago-Diaz5 , Júlia Perera-Bel1 , Pau Berenguer-Molins1 , Monica Martinez Gallo6 , Andrea Martin-Nalda7 , Encarna Varela8,9 , Marta Garrido-Pontnou10 , Fabio Grassi4 , Francisco Guarner8,9 , Saurabh Mehandru3 , Lucia Márquez-Mosquera11 , Ramit Mehr2** , Andrea Cerutti1,3,12** , and Giuliana Magri1** Cytometry:Article Title: Expression of Human CEACAM Receptors Promotes Inflammation and Organ Damage During Systemic Candida albicans Infection in Mice Article Snippet: .. Cells were stained and analyzed on an Attune Acoustic Focusing Cytometer (Life Technologies, Thermo Fisher Scientific, Darmstadt, Germany) using the Attune software v2.1, as described in the respective figure legends with the following antibodies or the corresponding Software:Article Title: Expression of Human CEACAM Receptors Promotes Inflammation and Organ Damage During Systemic Candida albicans Infection in Mice Article Snippet: .. Cells were stained and analyzed on an Attune Acoustic Focusing Cytometer (Life Technologies, Thermo Fisher Scientific, Darmstadt, Germany) using the Attune software v2.1, as described in the respective figure legends with the following antibodies or the corresponding Immunolabeling:Article Title: Single-cell RNA sequencing reveals disease associated changes in brain endothelial cells in the 5XFAD mouse. Article Snippet: After tissue homogenization, dissociation, myelin/debris removal, and red blood cell lysis, cells were incubated with fluorescently conjugated antibodies against CD31 (0.1 μg/mL, Invitrogen 170311-82) and CD45 (0.1 μg/mL, BD Pharmingen 553081) diluted in 0.5% BSA (w/v) (Research Products International A30075100.0) in Dulbecco’s Phosphate-Buffered Saline (DPBS, Gibco 141190-144) for 30 minutes at 4°C. .. After immunolabeling, cells were rinsed with DPBS containing 0.5% BSA and resuspended in DPBS containing 0.5% BSA with 4’6-Diamidino-2-phenylindole (DAPI, 0.2μg/mL, Sigma Aldrich D9542) or without DAPI for single stained compensation controls and Article Title: Single-cell RNA sequencing reveals disease associated changes in brain endothelial cells in the 5XFAD mouse Article Snippet: After tissue homogenization, dissociation, myelin/debris removal, and red blood cell lysis, cells were incubated with fluorescently conjugated antibodies against CD31 (0.1 μg/mL, Invitrogen 170311-82) and CD45 (0.1 μg/mL, BD Pharmingen 553081) diluted in 0.5% BSA (w/v) (Research Products International A30075-100.0) in Dulbecco’s Phosphate-Buffered Saline (DPBS, Gibco 141190-144) for 30 min at 4 °C. .. After immunolabeling, cells were rinsed with DPBS containing 0.5% BSA and resuspended in DPBS containing 0.5% BSA with 4’6-Diamidino-2-phenylindole (DAPI, 0.2 μg/mL, Sigma Aldrich D9542) or without DAPI for single stained compensation controls and Flow Cytometry:Article Title: Immunomolecular and reactivity landscapes of gut IgA subclasses in homeostasis and inflammatory bowel disease. Article Snippet: Sonia Tejedor Vaquero1* , Hadas Neuman2* , Laura Comerma1,5 , Xavi Marcos-Fa1 , Celia Corral-Vazquez1 , Mathieu Uzzan3 , Marc Pybus1 , Daniel Segura-Garzón1 , Joana Guerra1 , Lisa Perruzza4 , Roser Tachó-Piñot1 , Jordi Sintes1 , Adam Rosenstein3 , Emilie K. Grasset3 , Mar Iglesias5 , Monica Gonzalez Farré5 , Joan Lop5 , Maria Evangelina Patriaca-Amiano5 , Monica Larrubia-Loring5 , Pablo Santiago-Diaz5 , Júlia Perera-Bel1 , Pau Berenguer-Molins1 , Monica Martinez Gallo6 , Andrea Martin-Nalda7 , Encarna Varela8,9 , Marta Garrido-Pontnou10 , Fabio Grassi4 , Francisco Guarner8,9 , Saurabh Mehandru3 , Lucia Márquez-Mosquera11 , Ramit Mehr2** , Andrea Cerutti1,3,12** , and Giuliana Magri1** Magnetic Cell Separation:Article Title: A novel agarose-free, standardized generation and versatile ECM characterization of decellularized scaffolds from normal and fibrotic human lung tissue Article Snippet: .. Fibroblasts (500,000 cells; passage 7) were resuspended in 100 μL MACS buffer (0.5% (w/v) BSA, 2 mM EDTA) containing fluorophore-conjugated antibodies against CD90 (FITC Anti-human CD90 (Thy-1), clone REA897 Miltenyi Biotec, Cat. No.: 130-114-859), CD31 (APC Anti-human CD31, clone REA1028, Miltenyi Biotec, Cat. No.: 130-117-226), CD45 (VioBlue Anti-human CD45, clone REA747, Miltenyi Biotec, Cat. No.: 130-110-637) each 1:50 dilution or Recombinant:Article Title: A novel agarose-free, standardized generation and versatile ECM characterization of decellularized scaffolds from normal and fibrotic human lung tissue Article Snippet: .. Fibroblasts (500,000 cells; passage 7) were resuspended in 100 μL MACS buffer (0.5% (w/v) BSA, 2 mM EDTA) containing fluorophore-conjugated antibodies against CD90 (FITC Anti-human CD90 (Thy-1), clone REA897 Miltenyi Biotec, Cat. No.: 130-114-859), CD31 (APC Anti-human CD31, clone REA1028, Miltenyi Biotec, Cat. No.: 130-117-226), CD45 (VioBlue Anti-human CD45, clone REA747, Miltenyi Biotec, Cat. No.: 130-110-637) each 1:50 dilution or Suspension:Article Title: Brain capillary endothelial-like cells show altered barrier functionality and reduced transport of amyloid β in late-onset Alzheimer disease Article Snippet: Next, 1 × 10 6 cells were permeabilised and fixed with a FoxP3 staining buffer set (Miltenyi Biotec, #130-093-142) as recently described [ ]. .. The obtained cell suspension was incubated on ice for 30 min using the following fluorescence-labelled primary antibodies and Incubation:Article Title: Brain capillary endothelial-like cells show altered barrier functionality and reduced transport of amyloid β in late-onset Alzheimer disease Article Snippet: Next, 1 × 10 6 cells were permeabilised and fixed with a FoxP3 staining buffer set (Miltenyi Biotec, #130-093-142) as recently described [ ]. .. The obtained cell suspension was incubated on ice for 30 min using the following fluorescence-labelled primary antibodies and Fluorescence:Article Title: Brain capillary endothelial-like cells show altered barrier functionality and reduced transport of amyloid β in late-onset Alzheimer disease Article Snippet: Next, 1 × 10 6 cells were permeabilised and fixed with a FoxP3 staining buffer set (Miltenyi Biotec, #130-093-142) as recently described [ ]. .. The obtained cell suspension was incubated on ice for 30 min using the following fluorescence-labelled primary antibodies and Control:Article Title: Brain capillary endothelial-like cells show altered barrier functionality and reduced transport of amyloid β in late-onset Alzheimer disease Article Snippet: Next, 1 × 10 6 cells were permeabilised and fixed with a FoxP3 staining buffer set (Miltenyi Biotec, #130-093-142) as recently described [ ]. .. The obtained cell suspension was incubated on ice for 30 min using the following fluorescence-labelled primary antibodies and |